I would recommend that you use the manual focus mode. A camera is not always able to realize focus because of issues such as
- low light
- objects at different distances
- low contrast
- objects in scene move
- bright light source relative to overall lighting
If the camera cannot achieve good focus for the length of time that the signal shorts the focus pin to ground then the shutter will not be actuated if the lens is in the autofocus mode. So when achieving good focus is possible, you may need to give the camera more time.
You can realize better critical focus by using the joystick to move the white rectangle to an area that you want to focus on while live view is enabled. ( You enable live view by pressing the button at the center of the large dial. It is necessary to enable live using the menu as the default is that live view is disabled ) Then press the small + button to magnify the screen to 5x and then again to 10x. This should allow you to realize a better critical focus that you can achieve using AF.
Note: You are not able to access the menu system on Canon or Nikon when the remote shutter cable or USB or plugged in
Note: The Nikon cameras have focus motor in the camera body as well as some lenses. You must switch the switch to M for manual focus as well as any lens switch for AF/M focus.
Note: The Nikon live view mode is on the dial at the top left of the camera body for cameras with the 10 pin remote shutter connector.
An exception to setting a Canon lens to AF would be if you are using a laptop or netbook with Breeze Systems remote control software and want to focus bracket along with exposure bracketing.